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JNJ-6204

2D structure
Target CSNK1D  CSNK1E 
Targeted domain Kinase domain (active site)
Mode of action Inhibitor (type 1, ATP competitive)
Control JNJ-0293
Recommended cellular usage concentration ≤ 1 µM
In vivo use Yes
Synonyms JNJ-78386204
Donated by Johnson & Johnson


Probe criteria


Inhibitor/agonist potency: goal is < 100 nM (IC50, Kd) Surpasses criterion: CSNK1D: IC50 = 2.3 nM; CSNK1E: IC50 = 137 nM (purified Enzyme, ADP-GLO)
Selectivity within target family: > 30-fold Closely related kinases: CSNK1A1 (IC50 = 419 nM)
Panel of 370 kinases at 1 μM: clean; Closest hit: TNIK (IC50 = 1.6 μM)
Selectivity outside target family Coming soon.
On target cell activity for cell-based targets: goal is < 1 µM IC50/EC50 Surpasses criterion:CSNK1D: BRET whole cell binding assay: IC50 = 72 nM; PER2 translocation whole cell functional assay: IC50 = 542 nM
Control compound (100 times less potent than the probe) JNJ-0293: Purified Enzyme, ADP-GLO: IC50 = 6.9 μM; BRET whole cell binding assay: IC50 > 10 μM